ISHS Acta Horticulturae 988: V International Symposium on Acclimatization and Establishment of Micropropagated Plants
EFFECTS OF NITROGEN FORM AND RATIO ON IN VITRO SHOOT MULTIPLICATION AND GROWTH OF LOWBUSH BLUEBERRY (VACCINIUM ANGUSTIFOLIUM AIT.) |
| Authors: | J.P. Santiago, J.M. Smagula |
| Keywords: | micropropagation, plant nutrition, ammonium, nitrate, nitrogen |
| DOI: | 10.17660/ActaHortic.2013.988.14 |
| Abstract:
Lowbush blueberry plants grow best in acidic soil and prefer the ammonium (NH4+) form of nitrogen (N). A micropropagation study of lowbush blueberry in Canada with two media providing N in varying NO3- to NH4+ ratios reported that shoot multiplication was best on medium with NO3- as the sole source of N. To clarify this discrepancy regarding the N preference of lowbush blueberry we cultured single-node explants of two cultivars (‘CVAC 799’ and ‘CVAC 219’) on Z-2 tissue culture medium providing 15 meq of N with NO3- to NH4+ ratios of 1:0, 2:1, 1:2, 1:1, or 0:1. After 9 weeks on the treatment medium, single-bud explants were prepared from the plantlets in each treatment and then subcultured for another 9 weeks on fresh treatment medium with the same NO3:NH4 ratio on which it had been previously cultured. Shoot multiplication of ‘CVAC 799’ was greater when cultured on an equal proportion of NO3- and NH4+ than when cultured on a Vaccinium medium with a NO3- to NH4+ ratio of 2:1 that is used for many Vaccinium species. Shoot multiplication for ‘CVAC 219’ was greatest on medium with either the NO3- to NH4+ ratio of 2:1 or an equal ratio of NO3- to NH4+. The ratio of NO3- to NH4+ had little or no effect on shoot length or leaf number for either clone. Neither clone tolerated NO3- as the sole source of N. | |
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