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ISHS Acta Horticulturae 972: II International Symposium on Medicinal and Nutraceutical Plants

IN VITRO EVALUATION OF PSORALEN THROUGH NODAL CULTURES OF PSORALEA CORYLIFOLIA EXPOSED TO ELICITORS

Authors:   V. Agrawal, S.P.K. Chetri, B.M. Parast
Keywords:   Psoralea corylifolia, psoralen, secondary metabolites, inorganic elicitors, organic elicitors, precursors of psoralen
DOI:   10.17660/ActaHortic.2013.972.1
Abstract:
Present investigation highlights the evaluation of an important but less explored anticancerous bioactive compound, psoralen from Psoralea corylifolia. HPLC analysis of in vivo plant parts such as roots, nodes and leaves revealed that the quantity of psoralen was optimum in nodal parts. In vitro enhanced biosynthesis of psoralen was achieved in the nodal cultures (mature and juvenile) of Psoralea corylifolia, employing various elicitors (organic) including precursors of psoralen synthesis pathway. HPLC analysis of the crude extract of mature and juvenile nodal cultures revealed that the former was more competent to synthesize psoralen (1574 g/g fresh wt.) than juveniles (474 g/g fresh wt.). Addition of different elicitors to culture media substantially improved the biosynthesis of psoralen. The quantity of psoralen was found to be enhanced at all the levels of the treatments but the improvement of psoralen was dose dependent and elicitor specific. Amongst amino acids, proline induced optimum synthesis in either of the case. Amongst precursors, such as umbelliferone, cinnamic acid and NADPH, the former two proved promotory. Amongst all the elicitors employed as a whole, cinnamic acid at 5 mg/L and proline at 50 mg/L induced the optimum synthesis of psoralen in case of mature and juvenile nodal explants, respectively. This is our first report of optimizing psoralen biosynthesis in the organ cultures through elicitors.

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