ISHS Acta Horticulturae 935: XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on New Developments in Plant Genetics and Breeding
EMBRYO RESCUE OF SOLANUM LYCOPERSICUM × S. PERUVIANUM TOMATO CROSS FOR TOCV RESISTANCE |
| Authors: | C.L. Encina, E. Carmona-Martin , R. Fernández-Muńoz |
| Keywords: | in vitro, breeding, tomato chlorosis virus, virus tolerance, interspecific hybrids, incompatibility |
| DOI: | 10.17660/ActaHortic.2012.935.12 |
| Abstract:
The tomato line ‘EELM-802’ shows strong resistance to Tomato chlorosis virus (ToCV). Attempts to cross this line with a susceptible parent in order to study genetics of the resistance have failed. ‘EELM-802’ was derived by selfing and selection from ‘IAC-CN-RT’, a population obtained by embryo rescue in Brazil from a cross between the cultivated tomato species (Solanum lycopersicum L.) and the wild relative S. peruvianum L. Clearly, the unilateral incompatibility described to hinder the crosses between these two species was still present in the ‘IAC-CN-RT’ hybrid population leading to early abortion of embryos. Pollen from ‘EELM-802’ was used to pollinate the susceptible cultivar ‘Moneymaker’, and proembryos were collected from developing fruits 17-24 days after pollination. Rescue of immature embryos was obtained from proembryos 21 days after pollination. One of the regenerated plantlets showed intermediate morphological charac-ters between ‘Moneymaker’ and ‘EELM-802’. True hybrid nature of that plant was further confirmed by using a codominant molecular marker showing polymorphism between the parents. Currently, the ‘Moneymaker’ × ‘EELM-802’ hybrid specimen is being selfed and backcrossed to obtain segregating generations for the study of ToCV-resistance inheritance and three stocks of clonal copies of the hybrid and parental genotypes are maintained in vitro. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
