ISHS Acta Horticulturae 908: I International Symposium on Cryopreservation in Horticultural Species
CRYOBANKING OF BANANA (MUSA SP.) GERMPLASM IN INDIA: EVALUATION OF AGRONOMIC AND MOLECULAR TRAITS OF CRYOPRESERVED PLANTS |
| Authors: | A. Agrawal, R.K. Tyagi, R. Goswami, S. Uma, M.S. Saraswathi, P. Durai |
| Keywords: | vitrification, morphological evaluation, simple sequence repeats, genetic stability |
| DOI: | 10.17660/ActaHortic.2011.908.14 |
| Abstract:
Conservation of banana diversity is imperative as anthropogenic activities coupled with threats of climate change are causing fast depletion. Cryopreservation, using in vitro-derived shoot meristems, is a safe long-term conservation method for Musa germplasm. The aim of the present study was to compare the performance of cryopreserved banana germplasm of cultivar ‘Sommarani Monthan’ (AAB, Monthan subgroup) for agronomic and molecular traits (using simple sequence repeats; SSR) with that of its parental type. Experiment included cryopreserved germplasm, cryopreservation control, in vitro-conserved plants, and natural sucker-raised plants. Genetic stability of original mother plants maintained in the field genebank, in vitro-conserved plants, PVS2-treated plants and cryopreserved plants was assessed using 12 agronomic traits and nine SSR primers. Shoot tip cultures were conserved on MS + BAP (10 µM) + IAA (1 µM) + 3% sucrose + 0.8% agar medium for 12 months with 1.2 shoots/subculture. In the in vitro-derived shoot meristem cultures processed for cryopreservation, the mean shoot regeneration in PVS2-treated (without LN exposure) was 56.5%. In the LN-treated explants, 41% shoot regeneration was obtained in explants treated with PVS2 for 120 min, which could be recovered successfully as complete plantlets. Results indicated that no significant differences existed for the major growth and yield parameters analyzed, except in one plant. In one of the plants raised from cryopreserved meristems, the fruit colour was found to be green instead of ash green (as in the control plants). Among the nine primer pairs tested, seven primers pairs namely AGMI-33,34, AGMI-35,36, AGMI-67,68, AGMI-93,94, AGMI-95,96, AGMI-129,130 and MbSSR 1-149 amplified products resulting in discrete repeatable amplicons, wherein a total of 13 alleles were identified with a mean of 1.86 alleles per primer. All the alleles were found to be monomorphic. The SSR primer pairs tested also did not show any variation for the green fruited variant as compared with control plants. | |
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