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ISHS Acta Horticulturae 840: I International Jujube Symposium

GENETIC ANALYSIS OF ZIZIPHUS JUJUBA 'HUIZAO' USING ISSR MARKERS

Authors:   J.D. Li, H.T. Bi, H.T. Li, Z.S. Li, J.C. Feng
Keywords:   Ziziphus jujuba ¿Huizao¿, ISSR, genetic analysis
DOI:   10.17660/ActaHortic.2009.840.15
Abstract:
To evaluate the genetic structure of Ziziphus jujuba ‘Huizao’, which is one of the most popular cultivated jujube cultivar in Henan province, we collected 117 sample trees from the distributing area of Z. jujuba ‘Huizao’. DNA was extracted from the mature leaves and a most efficient ISSR-PCR reaction system was established. Ten most efficient primers were selected from 70 ISSR primers. Ninety-six patterns were amplified and 76 of them (79.17%) were polymorphic patterns. Gel analysis by POPGEN 1.32 software showed that the mean standard genetic distance was 0.3951. The observed number of alleles (Na) was 1.7917, effective number of alleles (Ne) was 1.2941. Shannon’s information index (I) was 0.2903, and the Nei’s genetic diversity (He) was 0.1834. The result showed a high genetic diversity level among the population, thus great breeding potential existed in the Z. jujuba ‘Huizao’. Cluster and principle coordinate analysis shows that the population can not be separated significantly. There is no co-relationship between geographic and genetic distance. We suggest collecting some sample trees by the phenotypic variation for germplasm conversation and new cultivar selection.

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