ISHS Acta Horticulturae 812: III International Symposium on Acclimatization and Establishment of Micropropagated Plants
IN VITRO ROOTING AND ACCLIMATISATION OF SERVICE TREE (SORBUS DOMESTICA L.) |
| Authors: | E. Caboni, M. Tonelli, C. Damiano |
| Keywords: | indole-3-butyric acid, micropropagation, vermiculite |
| DOI: | 10.17660/ActaHortic.2009.812.82 |
| Abstract:
In vitro propagated shoots, obtained from axillary buds were treated, to induce rooting, with 2 mg L-1 IBA for all the rooting phase, quick immersion of the basal part of microcuttings in 2 g L-1 IBA solution for 1 or 10 min and transferring to a PGR-free agarised medium; an immersion of basal parts in 20 g L-1 sucrose solution containing increasing concentrations (0-100 mg L-1) of IBA for 1 or 5 days and transferring to a PGR-free agarised medium or an immersion of basal parts of the microcuttings in 20 g L-1 sucrose solution containing 80 mg L-1 IBA for 1 day and transferring to a PGR-free medium with or without agar and with or without vermiculite. The best response in terms of rooting and acclimatisation was obtained with an induction in 80 mg L-1 IBA solution applied for 1 day and a transferring to a liquid medium with vermiculite. This protocol allows to obtain 80% of rooted microcuttings and a 98% of survival after acclimatisation. | |
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