|Authors: ||A. Sasaki, T. Shimane, K. Azegami, T. Matsuura, Y. Inoue|
|Keywords: ||fire blight, epiphytic bacteria, Enterobacteriaceae, Pseudomonas, 16S ribosomal DNA sequence, immature pear fruit |
A total of 278 bacteria were isolated from Japanese pear flowers in three areas to determine their genera and pathogenicity.
Partial 16S ribosomal DNA sequence similarity and colony count revealed that dominant genera on Japanese pear flowers consisted of Pseudomonas spp. and Enterobacteriaceae (Erwinia spp., Pantoea spp. and Rhanella spp.), followed by Bacillus spp. in all the sampling areas but that bacterial flora varied in quantity and quality depending on the time after flowering and individual trees.
Pathogenicity of 174 isolates was determined by inoculation on sliced, immature pear fruits.
Several isolates of Pseudomonas and Bacillus caused slight symptoms (i.e., black discoloration and brown soft rot on inoculated surface), but they were distinguishable from symptoms caused by E. amylovora.
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