|Authors: ||A.M. Bocsanczy, S.V. Beer, N.T. Perna, B. Biehl, J.D. Glasner, S.W. Cartinhour, D.J. Schneider, G.A. DeClerck, M. Sebaihia, J. Parkhill, S. Bentley|
|Keywords: ||microbial genomes, enterobacteria, Ea273, annotation, ASAP, plant pathogen|
The entire genome of E. amylovora, strain Ea273, was sequenced at the Sanger Institute.
A manuscript reporting the total sequence and its annotation is in preparation by authors in three locations.
The genome of Ea273 is ca. 3.8 mbp, which is small compared with other enterobacteria including non-pathogenic E. coli K-12, and it shows signs of erosion due to many pseudogenes and "lost" genes.
It also has a limited number of homologs with proteins associated with the type three secretion system (T3SS) of other sequenced Gram-negative plant pathogens.
Analysis of a preliminary annotation identified several potential novel virulence factors, including two novel T3SS islands.
The genome sequence can be accessed at http://www.sanger.ac.uk/Projects/E_amylovora/. The preliminary annotation and sequence can be accessed on request and registration at: https://asap.ahabs.wisc.edu/asap/logon.php.
The ASAP database, maintained at the University of Wisconsin, Madison, is an extensive compendium of information relative to microbial genomes, along with tools that facilitate comparisons of the genome of Ea273 with that of other sequenced bacteria.
To increase the value of the genome to the fire blight community and others, additional biological annotation is solicited.
Curation of the genome will be done both at UW-Madison by Nicole Perna and at Cornell by Ana Maria Bocsanczy.
In the following few years, we hope that the genome sequence will facilitate greater understanding of fire blight at both the molecular genetic and practical disease control levels.
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