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ISHS Acta Horticulturae 764: XXVII International Horticultural Congress - IHC2006: International Symposium on Plant Biotechnology: From Bench to Commercialization

DETECTION OF STRAWBERRY MOTTLE VIRUS BY RT-PCR

Authors:   H. Yang, Z. Zhang, H. Dai, H. Li
Keywords:   strawberry mottle virus, in vitro plants, detection limit, RT-PCR
DOI:   10.17660/ActaHortic.2007.764.15
Abstract:
Strawberry mottle virus (SMoV) is one of the most economically important viral pathogens infecting strawberries (Fragaria spp.) worldwide. Losses of fruit yield and runner production in commercial strawberries due to infection by SMoV are high. In this study, detection of SMoV by reverse transcription-polymerase chain reaction in both in vitro plants and field-grown plants with a modified CTAB method for extracting total RNA was described. Forty-two field strawberry plants of eighteen strawberry cultivars were tested with the primer pair specific for the non-coding region of SMoV and eleven samples showed positive results. The in vitro plants had lower detection limits than field samples. Large coat protein regions of four Chinese isolates of SMoV were amplified, and testified by sequencing. Sequence analysis for 20 isolates showed nucleic acid and amino acid identities ranged from 76.8 to 99.7% and 89.8 to 100%, respectively.

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