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Authors: | N. Junka, C. Wongs-Aree, S. Kanlayanarat, K. Thanomchit |
Keywords: | Aerides multiflora, Aerides rosea, cyanidin, highly acylated anthocyanins, sequential extractions |
DOI: | 10.17660/ActaHortic.2007.755.76 |
Abstract:
Anthocyanins, a group of plant pigments, play a crucial role in colour appearance of orchid flowers.
Characterisation of anthocyanins accumulating in Aerides multiflora Roxb. and Aerides rosea Lodd. ex Lindl. & Paxt. flower was investigated using sequential extraction techniques.
The anthocyanidin profiles from Aer. multiflora and Aer. rosea were compared to the profiles of reference anthocyanidins extracted from concord grape, strawberry, butterfly pea, and red cabbage.
Cyanidin revealed to be a predominant predecessor of other anthocyanins distributing in the flower of both species.
Anthocyanin structures of Aer. multiflora and Aer. rosea flower were conjugated with sugars and acids, exhibiting complex acylated anthocyanin features as shown in bathochromatic effects of two maximum absorption peaks at 544 nM and 584 nM when incubated in pH 4.5. The absorption spectra of anthocyanins in buffer pH 4.5 were disappeared after use of bisulfite treatments or deacylation by saponification reactions.
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