ISHS Acta Horticulturae 731: III International Symposium on Cucurbits
DEVELOPMENT OF A TRIVALENT TRANSGENIC WATERMELON BH-1 RESISTANT TO VIRUS INFECTIONS |
| Authors: | N. Sheng-Niao, H. Xue-Sen, W. Sek-Man, Y. Jia-Lin, Z. Fu-xin, W. Sen-you, Z. Guang-Ming, S. Fan-Sheng, C. Xiao-Yu, C. Yong-Gang, X. Yan, Z. Fang, W. Xiao-Hong |
| Keywords: | watermelon, virus diseases, gene transformation, resistance to virus infection |
| DOI: | 10.17660/ActaHortic.2007.731.8 |
| Abstract:
By using the coat protein gene of WMV-2, and replicase genes of ZYMV and CMV, a trivalent expression vector was constructed for transformation of watermelon plants with Agrobacterium tumefaciens. The transgenes were confirmed in progenies of T1, T2 and T3 generations by PCR-Southern blot analysis. By mechanical inoculation in greenhouse and field trial, the transgenic lines showed resistance to virus infections in relatively high level and the resistant transgenic line BH-1 was selected successfully. | |
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