ISHS Acta Horticulturae 689: VII International Symposium on Grapevine Physiology and Biotechnology
CLONAL POLYMORPHISM IN THE RED WINE CULTIVARS ´CARMENÉRE´ AND ´CABERNET SAUVIGNON´ |
| Authors: | X. Moncada, L. Muñoz, D. Merdinoglu, M.H. Castro, P. Hinrichsen |
| Keywords: | Vitis vinifera, molecular markers, SSR, AFLP. |
| DOI: | 10.17660/ActaHortic.2005.689.63 |
| Abstract:
The cultivar ‘Carmenère’ is originated from the Médoc in France. Its presence was dramatically reduced after the phylloxera crisis during the second half of the 19th century. During the last decade no more than 10 ha were registered in France. In contrast, ‘Carmenère’ was re-discovered in 1994 in Chile, where it was confused with ‘Merlot’. In the present study we compared the polymorphic level of Carmenère and Cabernet Sauvignon clones by using microsatellite and AFLP markers. Fifty-six ‘Carmenère’ clones from clonal evaluation plots in Chile as well as nine clones from France and one from Italy were analyzed. Only two out of 21 microsatellite markers assayed were found polymorphic. VVMD7 locus exhibited two allelic forms, grouping 67% and 33% of the clones, respectively. Instead, VMC5g7 locus showed only one mutated allele in one clone. AFLP analysis of the Chilean Carmenère clones showed 2.3% polymorphism. In contrast, an analysis of 75 ‘Cabernet Sauvignon’ clones with 91 microsatellite markers found 17 polymorphic loci, and an analysis of 46 of these clones with AFLP found 5.9% clonal polymorphism. These results suggest that the genetic diversity in ‘Carmenère’ clonal germplasm is low, in comparison to the more diverse germplasm of Cabernet Sauvignon clones. | |
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