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ISHS Acta Horticulturae 626: XXVI International Horticultural Congress: Berry Crop Breeding, Production and Utilization for a New Century

FLAVONOID CONTENT AND COMPOSITION IN LEAVES AND BERRIES OF SEA BUCKTHORN (HIPPOPHAE SPP.) OF DIFFERENT ORIGIN

Authors:   B. Barl, L. Akhov, D. Dunlop, S. Jana, W.R. Schroeder
Keywords:   germplasm, medicinal value, HPLC, column chromatography, flavonols, fingerprinting
DOI:   10.17660/ActaHortic.2003.626.55
Abstract:
Sea buckthorn (Hippophae spp.) is a hardy, deciduous shrub producing small berries, with wide but fragmented distribution in Eurasia between 27 and 69N latitude and 7W and 122 E longitude. It has had many diverse uses, from controlling soil erosion to being a source of horse fodder, nutritious foods, drugs and skin care products. In Canada in recent years, sea buckthorn has been recognized as a versatile nutraceutical crop with great economic potential, which has led to development of over 400 acres of sea buckthorn orchards. Flavonoids, a large group of phenolic compounds with several well documented biological activities beneficial to human health, are one of several important constituents of sea buckthorn leaves and berries. High flavonoid content could be used as a desirable character in selection of sea buckthorn suitable for Canadian prairie. The objective of this study was to determine total flavonoid content and flavonoid profile in leaves and berries of Hippophae species originating from China, Russia, Finland and Canada, collected from the sea buckthorn germplasm nursery at the PFRA Shelterbelt Centre in Indian Head, Saskatchewan. Three species (H. rhamnoides, H. gyantensis, H. neurocarpa) and four subspecies of H. rhamnoides: rhamnoides, sinensis, turkestanica and mongolica ('Indian Summer') were investigated. Results from a three-year study of leaf and berry samples indicated that flavonoid content, expressed as isorhamnetin equivalents, ranged from 0.83% to 2.00% in air-dried leaves, and 0.18% to 0.56% in lyophilized berry flesh. 'Indian Summer' selection R-C4 Rafferty contained the highest amount of flavonoids in both leaves (1.7% to 2.0%) and berries (0.41% to 0.49%). Flavonoid content was the lowest in leaves of H. rhamnoides ssp. rhamnoides. The RP-HPLC flavonoid profiling revealed complex flavonoid composition consisting of at least 13 distinct compounds in leaves and 19 in berries. Flavonoid composition differed at the species, subspecies and variety level and was believed to be genetically and environmentally controlled.

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