ISHS Acta Horticulturae 597: International Conference on Medicinal and Aromatic Plants (Part II)
EFFECT OF MAGNESIUM ON THE GROWTH AND ALKALOID PRODUCTION OF HAIRY ROOT CULTURES |
| Authors: | H. Hank, I. Lásló, I. Bálványos, I. Kursinszki, G. Kovács, E. Szöke, E. Töth |
| Keywords: | Atropa belladonna, hairy root cultures, hyoscyamine, scopolamine, magnesium |
| DOI: | 10.17660/ActaHortic.2003.597.39 |
| Abstract:
In vitro cultures of Atropa belladonna L. (Solanaceae) cultivated on a half dilution MS (Murashige and Skoog) medium containing 3% sucrose, was infected by microinjection with Agrobacterium rhizogenes strain R1601. The hairy roots formed within 2 months were isolated, subcultured on B5 (Gamborg) medium and selected from the point of view of growth and tropane alkaloid production. Determination of alkaloids was performed by HPLC methods. Atropine, scopolamine and 6-OH-hyoscyamine were identified and measured by this technique. We have studied the effect of MgSO4 on the growth and alkaloid production of genetically modified hairy roots as well. | |
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