ISHS Acta Horticulturae 528: VII International Symposium on Grapevine Genetics and Breeding
PROANTHOCYANIDINS PRODUCTION IN TISSUE CULTURE: REGULATION OF DIHYDROFLAVONOL REDUCTASE PROMOTER-GUS-INTRON FUSION IN GRAPES |
| Authors: | R. Gollop, Y. Eshdat, A. Perl, V. Tsolova |
| Keywords: | Proanthocyanidins, DFR, gene regulation, transformation, GUS |
| DOI: | 10.17660/ActaHortic.2000.528.57 |
| Abstract:
The dihydroflavonol reductase (DFR) promoter from Vitis vinifera cv. Red Globe was cloned, and fused to GUS-Intron as a reporter gene. The fusion construct was transformed to grape cell suspension of Vitis vinifera cv. Gamay Red and to embryogenic cell line of the cultivar Superior Seedless. Positive transformants expressing the DFR promoter-GUS-Intron gene, as analyzed by GUS activity, were obtained from both cultivars. Growth culture conditions for induction of procyanidin production were established. Procyanidin production was induced in callus derived from the cultivar Gamay Red by illumination of 40 (μE m-2 sec-1, in the presence of kinetin and  - Naphthaleneacetic acid.
An optimal concentration of riboflavin, casein hydrolyzate and sucrose was determined for procyanidin production.
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