ISHS Acta Horticulturae 517: XXV International Horticultural Congress, Part 7: Quality of Horticultural Products
LEVEL OF FLAVONOLS IN WILD AND CULTIVATED ALLIUM SPECIES |
| Authors: | M. Horbowicz, T. Kotlinska |
| Keywords: | Allium, accessions, cultivars, flavonols, kaempferol, landraces, myricetin, quercetin |
| DOI: | 10.17660/ActaHortic.2000.517.47 |
| Abstract:
Levels of quercetin, kaempferol and myricetin in wild and cultivated species of Allium were investigated. The flavonols were determined in nine wild Allium species (A. ledebourianum, A.galanthum, A. altaicum, A. ampeloprasum, A. caesium, A. proliferum, A. fistulosum, A. nutans, A. vavilovii), nine landraces of shallot (A. cepa group aggregatum), in ten advanced cultivars of onion, and in cultivated garlic and leek. Leaves of one shallot landrace, bunching onion, two onion and one leek cultivars were analysed as well. The accessions of wild Allium species and shallot were originated from collection of plant Genetic Resources Lab. The flavonols were determined after acid hydrolysis of its glycosides, which were extracted in 60% of ethanol. Analyses were carried out using a HPLC equipped with a reversed phase RP-18 column, and UV detector set at 370 nm. The flavonols were eluted with methanol:water (55:45, v/v) contained 0.2% of orthophosphoric acid. Bulbs of A. ledebourianum, A. ampeloprasum, A. caesium, A. nutans, cultivated leek and garlic, and one onion cultivar (Albion F1) contained very low (below 20 mg/kg of fresh weight, FW) or traces (below 2 mg/kg FW) of quercetin and kaempferol. In rest of examined species level of quercetin ranged from 147 to 828 mg/kg FW, and kaempferol from traces to 173 mg/kg FW. Bulbs of shallot landraces and onion cultivars contained quercetin only. Tissues of A. galanthum, A. altaicum, A. proliferum, A. fistulosum, A. vavilovii as well as leaves of onion, bunching onion and shallot contained both quercetin and kaempferol. In leaves of analysed species the predominant flavonol form was kaempferol, that reached highest level 325 mg/kg FW in bunching onion. | |
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