ISHS Acta Horticulturae 501: II WOCMAP Congress Medicinal and Aromatic Plants, Part 2: Pharmacognosy, Pharmacology, Phytomedicine, Toxicology
DETERMINATION OF ANDROGRAPHOLIDE FROM ANDROGRAPHICS PANICULATA IN SERUM OF RATS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY |
| Authors: | R.A. Burgos, R.A. Schroeder, J.L. Hancke |
| Keywords: | Andrograhis paniculata, lactone, rat serum, HPLC, pharmacology |
| DOI: | 10.17660/ActaHortic.1999.501.10 |
| Abstract:
A simple method for analysis of andrographolide in the serum of rats by HPLC and photodiode array detection was developed. Andrographolide a diterpene lactone was extracted with acetone (4:1) and then analyzed using a RP-C18 lichrospher reversed phase column (4x125mm). A mobile phase consisting of 26% acetonitrile and 0.5% of phosphoric acid, at a flow rate of 1.1 mL/min, at 228 nm was used. The percentage of recovery of andrographolide ranged between 70–80% and the absolute detection limit in serum was 6.4 ng. | |
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