|Authors: ||R.V. Nikolova, N. Lall, A.J.N. Bosa|
|Keywords: ||Gamborg's B-5 medium, Impatiens flanaganae, Indoleacetic acid (IAA) in-vivo propagation, in-vitro propagation, Kinetin, Murashige and Skoog (MS) medium|
Impatiens flanaganae is a rare plant with beautiful pink flowers that is found in a few known locations in South Africa where local people use its tubers for medicinal purposes.
Considering its limited distribution, horticultural and medicinal potential, the possibility of propagating the plant both in-vivo and in-vitro was studied.
The lateral shoots induced by light of Impatiens flanaganae proved to be a suitable source for a more efficient vegetative propagation than the natural one via tubers.
The cuttings rooted relatively easily using commercial rooting powder.
Modified Murashige and Skoog medium (MS) supplemented with 2 mg/l Indoleacetic acid (IAA) and 2 mg/l Kinetin was found to be effective for the production of multi-axillary shoots (10 to 12 per explant) in-vitro when apical and lateral shoot tips were used as explants.
Rooting was induced on MS medium containing 1mg/l IAA. Callus was successfully initiated only from leaf explants on both MS and Gamborg's B-5 media supplemented with vitamins and selected concentrations of IAA and/or 2,4-D in combination with Kinetin.
Current studies are being carried out with respect to plant regeneration from callus.
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