ISHS Acta Horticulturae 431: Tospoviruses and Thrips of Floral and Vegetable Crops
DETECTION AND IDENTIFICATION OF TOMATO SPOTTED WILT VIRUS AND IMPATIENS NECROTIC SPOT VIRUS IN PORTUGAL |
| Author: | D. Louro |
| DOI: | 10.17660/ActaHortic.1996.431.9 |
| Abstract:
Tomato spotted wilt virus (TSWV) was first reported in Portugal in 1991 and found to be associated with imported ornamental plants. Subsequently, it became an important pathogen on horticultural crops. The virus was found in more than 30 plant species, including vegetables, ornamentals, and weeds. Outbreaks of TSWV occurred in greenhouse crops infested with Frankliniella occidentalis. Lettuce, pepper, and tomato were the most severely affected. Since 1994, impatiens necrotic spot virus (INSV) has also been found in imported ornamental plants. The virus was detected in 10 plant species but is not yet established in Portugal. The identification of these two tospoviruses was based on symptomatology, bioassay, virion morphology, and serology. Enzyme-linked immunosorbent assays (ELISA) and tissue-print immunoassays were used to identify the viruses. In some cases, when ELISA tests gave negative or erratic results, TSWV antigens were readily detected in small cell clusters in the tissue. Preliminary tests with naturally infected gloxinia tissues revealed a different distribution of TSWV and INSV in tissues of plants infected with both viruses, thus the tissue-print immunoassay appears to be a useful tool for diagnosing TSWV and INSV. | |
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