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ISHS Acta Horticulturae 264: II International Protea Research Symposium

MICROPROPAGATION OF LEUCOSPERMUM

Author:   J.T. Kunisaki
DOI:   10.17660/ActaHortic.1990.264.4
Abstract:
When axillary bud explants of Leucospermum conocarpodendron X L. cuneiforme 'Hawaii Gold' were cultured in liquid medium of modified ½ MS inorganic salts, vitamins (0.25 mg/1 each of pyridoxine.HCl and nicotinic acid and 0.2 mg/1 thiamine.HCl), 0.2 mg/1 BA and 2% sucrose, they developed proliferative bodies. When placed on filter paper bridges in shoot differentiation medium, these proliferations developed shoots which were then further elongated on agar medium. Microcuttings rooted in vitro were established under greenhouse conditions.

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