ISHS Acta Horticulturae 235: XIV International Symposium on Fruit Tree Virus Diseases
RECOVERY OF VIRUS-FREE PEACH TREES FROM SELECTED CLONES BY SHOOT-TIP GRAFTING IN VITRO |
| Authors: | J. Juárez, J.M. Arregui, E. Camarasa, M. Cambra, G. Llácer, C. Ortega, V. Ortega, L. Navarro |
| DOI: | 10.17660/ActaHortic.1989.235.9 |
| Abstract:
Peach cultivation in the Murcia region in Spain occupies more than ten thousand hectares. About 90% of this hectarage is planted with local varieties that produce high quality fruits, but low yields. More than 85% of peach trees are infected with apple chlorotic leaf spot virus (ACLSV) and 14% are infected with prunus necrotic ring spot virus (PNRSV). Recovery of virus-free peach trees was attempted by shoot-tip grafting in vitro (STG). Three sources of shoot tips were used: a) Shoots produced by budsticks forced at 30° C. b) Actively growing vegetative shoots from plants grown in a warm glasshouse (25–32° C). c) Actively growing vegetative shoots from plants grown in a growth chamber for 9 to 36 days at 35/30° C (16 h day/8 h night). ACLSV was very easy to eliminate by STG, and all the micrografted plants obtained from budsticks forced at 30° C were free of this virus. On the other hand, elimination of PNRSV by STG was significantly affected by the shoot tip source and size. Using plants grown in the growth chamber at 35° C day/30° C night, more than 90% of the plants derived from 0.6 to 1.0 mm long shoot tips were free of PNRSV. This treatment is a more efficient and quicker method for virus elimination than conventional thermotherapy. | |
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