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ISHS Acta Horticulturae 1309: IX International Strawberry Symposium

Whole genome assembly in a Japanese strawberry cultivar, 'Reikou', and comparison with wild Fragaria genomes

Authors:   K. Shirasawa, C. Chung, D. Boncan, H. Hirakawa, F. Maeda, T. Wada, T.F. Chan, S. Isobe
Keywords:   strawberry, Fragaria species, whole genome, de novo assembly
DOI:   10.17660/ActaHortic.2021.1309.26
Abstract:
Cultivated strawberry (Fragaria ananassa) is an allo-octoploid species, which chromosome number is 2n=8x=56. In this study, we report a chromosome-scale strawberry genome assembly of a Japanese cultivar. Genomes of diploid Fragaria species were re-sequenced and compared with the most similar chromosome-scale scaffolds to investigate possible progenitor of each subgenomes. The Illumina short reads derived from paired-end, mate pair and 10X Genomics libraries were obtained from a Japanese cultivar, 'Reikou'. The scafffolds assembled using Denovo MAGIC 3.0 consisted of 32,715 sequences with a total length of 1.4 Gb and N50 length of 3.9 Mb. The scaffolds were aligned onto the 'Reikou' S1 linkage maps with IStraw90 Axiom SaNP array and ddRAD-Seq for pseudomolecule construction.

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1309_25     1309     1309_27

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