ISHS Acta Horticulturae 1245: International Forum on Horticultural Product Quality
Mutation changes in flower colour and plant characteristics of Dendrobium by gamma irradiation |
| Authors: | P. Taywiya, A. Sawwa, T. Thiengnoi, S. Yooyongwech |
| Keywords: | ISSR marker, colour pattern, pseudobulb height |
| DOI: | 10.17660/ActaHortic.2019.1245.10 |
| Abstract:
Dendrobium is a popular orchid in commercial markets of Thailand. Release of new improved orchid cultivars remains a challenge in a competitive market. Various mutants of Dendrobium Sonia 'Earsakul' were generated by gamma irradiation. Orchid pseudobulbs (from 20 plants) were acutely (Cs-137) exposed to 0 to 200 Gray (Gy). The LD50 was detected at 50.8 Gy. White area of the flower centers and petals increased 1.83-fold especially after the 30 Gy treatment in the Mutation1 Vegetation1 (M1V1) plants. An ISSR marker with UBC844 primer clearly showed a different DNA profile during 400-600 bp, comparing with the control (0 Gy). Height of new pseudobulbs of the M1V1 plants exposed to 10, 20, and 30 Gy was around 20, 30, 40% less than control after 210 days, respectively. Dendrobium pseudobulbs of M1V1 plants treated with 30 Gy generated compact plants and enhanced white area in the flower. | |
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