ISHS Acta Horticulturae 1100: III International Symposium on Molecular Markers in Horticulture
CHARACTERISATION OF GENETIC DIVERSITY OF THE LITHUANIAN SOUR CHERRY (PRUNUS CERASUS L.) GENETIC RESOURCES USING MICROSATELLITE MARKERS |
| Authors: | R. Rugienius, J.B. Frercks, B. Siksnianienė, I. Stepulaitienė, G. Stanienė, D. Baniulis, V. Stanys |
| Keywords: | genetic variation, germplasm, microsatellite, heterozygosity, cultivars |
| DOI: | 10.17660/ActaHortic.2015.1100.14 |
| Abstract:
Thirty seven sour cherry (Prunus cerasus L.) accessions were evaluated using eight microsatellite (SSR) primer pairs in aim to characterise genetic diversity in the sour cherry collection of Institute of Horticulture Lithuanian Research Centre for Agriculture and Forestry (LRCAF IH). Seven to 20 alleles per primer pair were identified in the multiplex analysis set-up. The primer pair PCEGA34 was a most polymorphic and was able to discriminate 33 among the 37 sour cherry cultivars. A set of three microsatellite markers that distinguish all sour cherry cultivars used in the study was established. Cluster analysis of the microsatellite marker data established genetic relationship among the cultivars and distinguished cultivars with a different level of adaptation to climate conditions. | |
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