ISHS Acta Horticulturae 1010: III International Humulus Symposium
TECHNIQUE TO ASSESS GENE FUNCTION IN HOP (HUMULUS LUPULUS L.) - POWDERY MILDEW INTERACTIONS |
| Authors: | K. Oberhollenzer, E. Seigner, R. Eichmann, R. Hückelhoven |
| Keywords: | hop breeding, resistance, defence-related genes, transient transformation assay, transient-induced gene silencing (TIGS), Podosphaera macularis |
| DOI: | 10.17660/ActaHortic.2013.1010.6 |
| Abstract:
Improving resistance to powdery mildew (Podosphaera macularis ssp. humuli, Pm) is a major objective in hop breeding. To achieve this, classical breeding can be assisted by marker-assisted breeding or by transgenic approaches. For both these techniques, it would be of advantage to have DNA sequences available with confirmed function in the hop-powdery mildew interaction. Here, we report on the adaptation of a transient transformation assay for the functional assessment of defence- or susceptibility-related genes in hop as already available for barley and wheat. As a proof of concept, we performed transient-induced gene silencing (TIGS) of a putative hop powdery mildew susceptibility factor in hair cells, which resulted in a significantly reduced penetration success of the powdery mildew fungus. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
