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ISHS Acta Horticulturae 1010: III International Humulus Symposium

BIOSYNTHESIS OF FLAVONOIDS AND PHLOROGLUCINOLS: GENE EXPRESSION DURING HOP (HUMULUS LUPULUS L.) FLOWER DEVELOPMENT

Authors:   K.R. Häntzschel, E. Cevik, M. Collenberg, U. Born, A. Gatica-Arias, G. Weber
Keywords:   hop, flavonoids, phloroglucinols, flower development, gene expression
DOI:   10.17660/ActaHortic.2013.1010.5
Abstract:
Hop flavonoids and phloroglucinols are important secondary metabolites for brewing of beer as well as for medicinal uses. During female flower development these compounds are synthesized and finally accumulated in the lupulin glands of hop cones. Detailed information about the expression levels of genes with regard to the production of these substances might help in learning more about the underlying processes. Therefore, the following aspects were investigated: 1. Morphological characterization of different developmental stages of hop flowers. 2. Analysis of expression rates of relevant genes in biosynthetic pathways.
In this study, nine stages of female flower development were assigned to hop of the Tettnang variety. Furthermore, samples of each stage were harvested for RNA extraction. Finally, quantitative RealTime-PCR reactions were performed to measure expression levels of the following genes: cinnamate-4-hydroxylase (c4h), 4-coum-arate:CoA ligase (4cl), chalcone synthase (chs_h1), chalcone isomerase (chi), Valero-phenonesynthase (vps), prenyltransferase1 (hlpt1), and orthomethyltransferase1 (omt1).
Detailed morphological and molecular data of the different developmental stages of female hop flowers will be presented.

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