ISHS


Acta
Horticulturae
Home


Login
Logout
Status


Help

ISHS Home

ISHS Contact

Consultation
statistics
index


Search
 
ISHS Acta Horticulturae 994: I International Symposium on Date Palm

PRODUCTION OF SOMATIC EMBRYOS OF DATE PALM (PHOENIX DACTYLIFERA L.) FROM CELL SUSPENSION

Authors:   C. Selmani, D. Chabane, N. Bouguedoura
Keywords:   synchronous somatic embryos, plantlets, liquid medium, growth regulators
DOI:   10.17660/ActaHortic.2013.994.33
Abstract:
The main objective of this study is to obtain many individual and synchronous somatic embryos of date palm in liquid medium and reduce the time of their production. Friable calli were used to establish cell suspension. About 1 g of callus was chopped and cultivated in liquid culture medium containing 2.4-D and placed under rotary shaker (90 rpm) in light conditions at 27°C to initiate cell suspension. After two weeks, the cell-mixture is screened and filtered through a filter with a 1-mm diameter and transplanted in a liquid culture medium with the same composition. Many globular structures “pro-embryos” were observed and transferred in medium without growth regulators. This step is called pre-treatment of the cell suspension. To initiate embryos regeneration, the cell suspension was transferred to medium containing BAP at 5 µmoles for one week to develop leaf axis and transferring on the filter paper placed on a solid culture medium without growth regulators. Many young plantlets were obtained; their rooting was promoted by addition of ANA.
  • Article - full text (enhanced PDF format, 1925534 bytes, article contains substantial parts in French language)
  • How to cite this article
  • Translate

Download Adobe Acrobat Reader (free software to read PDF files)

994_32     994     994_34

URL www.actahort.org      Hosted by KU Leuven      © ISHS