|ISHS Acta Horticulturae 994: I International Symposium on Date Palm
MULTIPLICATION FROM INFLORESCENCE TISSUES: A POWERFUL PROCESS FOR MICROPROPAGATION OF SELECTED DATE PALM (PHOENIX DACTILYFERA L.) GENOTYPES
|Author: ||L. Abahmane |
|Keywords: ||Bayoud, floral buds, acclimatization, true-to-type, in vitro|
The national demand on date palm vitroplants has increased during the last decades.
Hence, Moroccan needs in this field, till the year 2020, are about 3 million plants.
Those plants will be used to rehabilitate palm groves devastated by Bayoud disease as well as to create new palm plantations.
To fulfill this plant demand, the use of all available rapid propagation techniques is of great importance.
Furthermore, the multiplication of improved genotypes should be privileged in this program.
However, most of the genotypes selected for their best fruit quality and their tolerance to Bayoud disease are represented in the nature by single trees and this makes their micropropagation from offshoots very difficult.
To overcome this problem, the use of tissues excised from young inflorescences remains the only way for micropropagation and wide diffusion of such genotypes.
Plant material is collected from inflorescences at their emergence and then well disinfected before transferring to culture media for vegetative buds initiation.
After many cycles of multiplication, complete plantlets can be regenerated and transferred to the greenhouse for acclimatization under controlled conditions.
This process has been developed and applied, to date, to micropropagate more than 14 genotypes, and this method can be used as a powerful technique to propagate rare or selected genotypes that have no more offshoots.
Plants produced by this technique and transferred to soil started to produce fruits in 2005. No abnormal growth or development was observed on those plants neither in the lab nor in the field.
In the present paper, the entire micropropagation process from inflorescence tissues and the main research achievements will be discussed.
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