ISHS Acta Horticulturae 988: V International Symposium on Acclimatization and Establishment of Micropropagated Plants
THE PROBLEMS IN ACCLIMATISATION OF IN VITRO MULTIPLIED PLANTS OF LAVANDULA ANGUSTIFOLIA MILLER UNDER FIELD CONDITIONS |
| Authors: | S. Mokhtarzadeh, M. Hajyzadeh, H.A. Ahmad, K.M. Khawar |
| Keywords: | Lavandula angustifolia, in vitro regeneration, acclimatization |
| DOI: | 10.17660/ActaHortic.2013.988.6 |
| Abstract:
English lavender (Lavandula angustifolia Miller) family Lamiaceae is an important plant widely grown in many parts of Europe, Africa, USA and Australia. It has beautiful violet coloured flowers, bluish green pointed leaves and grows in bush form to a height of 90 cm. Every year, a large foreign exchange is earned by essential oil export by many countries. Acclimatization of in vitro grown plants is very difficult needing an understanding of the growth habit and factors including the root apparatus of the plants to be acclimatized, surrounding temperature, humidity, soil template etc. responsible for plant growth under in vitro and ex vitro conditions. As the in vitro cultured plants are very sensitive to each of these even a very small lag in the look after of the tissue cultured plants can lead to damage and death. In line with this, the study aimed to acclimatize in vitro regenerated plantlets of L. angustifolia under field conditions. Initially the shoot regeneration was induced using variable concentrations of BAP-NAA on stem node explants followed by rooting on MS medium containing IBA. Thereafter, the plants were transferred to fields after a difficult procedure of acclimatization and optimization using hydroponic culture, sterilized sand, clay, peat and their mixture suggesting that every step after rooting, transfer of the plantlets to pots, acclimatization and transfer to fields, is very important. The study reports difficulties, problems and techniques to handle these substrates and their transfer to soil in open fields. The acclimatized plants are flourishing and growing well under Mediterranean continental type field conditions. | |
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