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ISHS Acta Horticulturae 961: VII International Symposium on In Vitro Culture and Horticultural Breeding

SOLANUM PENNELLII LA716 AS A SOURCE OF GENES IMPROVING IN VITRO ORGANOGENESIS IN CULTIVATED TOMATO

Authors:   F.N. Arikita, M.S. Azevedo, L.E.P. Peres, D.C. Scotton, A. Figueira
Keywords:   biotechnology, introgression lines, natural genetic variation, regeneration
DOI:   10.17660/ActaHortic.2012.961.43
Abstract:
In the present work we are reporting the high in vitro regeneration capacity of the tomato related wild species S. pennellii LA716, which enabled us to used a collection of 50 introgression lines (ILs), each containing small chromosomal segments of LA716 introgressed and mapped into the cultivar M82. We found a high shoot regeneration capacity for IL3-2, IL6-1, IL7-1 (7-2, 7-3), IL 8-3 (8-2), IL-9-1 (9-2) and IL10-2 (10-3), when 12-days-old cotyledon explants were cultivated in MS medium containing 5.0 μM BAP. This means that S. pennellii probably presents superior alleles for in vitro regeneration in such chromosomal segments. Since ILs 3-2, 7-1, 8-3, and 10-2 also presented enhanced root formation in MS medium containing 0.4 μM NAA, they may represent novel alleles controlling the competence to assume different cell fates, rather than the induction of a specific organ. The alleles discovered here will provide for the characterization and isolation of important genes for plant development studies and biotechnological applications.

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