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ISHS Acta Horticulturae 961: VII International Symposium on In Vitro Culture and Horticultural Breeding

IN VITRO CALLOGENESIS AND AGROBACTERIUM-MEDIATED TRANSFORMATION OF GLOBE ARTICHOKE

Authors:   B. Menin, A. Moglia, C. Comino, S. Lanteri, T.W.J.M. Van Herpen, J. Beekwilder
Keywords:   globe artichoke, callus culture, genetic transformation, Agrobacterium tumefaciens
DOI:   10.17660/ActaHortic.2012.961.34
Abstract:
Micropropagation techniques have been widely applied in globe artichoke (C. cardunculus L. var. scolymus), however, efficient protocols for the establishment of in vitro callogenesis and organogenesis, a pre-requisite for Agrobacterium-mediated genetic transformation, have not been set up so far. We developed an efficient protocol for callus induction from leaf explants of three globe artichoke genotypes of the varietal type ‘Romanesco’; after just one week culture callus formed with an efficiency close to 100%. Leaf explants were transformed with Agrobacterium tumefaciens Agl0 01-124 strain, harboring the binary vector pCAMBIA 2301 with the gene marker GUS, under the control of CaMV 35S promoter. After two weeks, about 30% of the calli obtained from infected leaf explants were positive to GUS assay.

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