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ISHS Acta Horticulturae 961: VII International Symposium on In Vitro Culture and Horticultural Breeding

USE OF AFLP-MARKERS FOR ESTIMATION OF THE INBREEDING LEVEL IN HELLEBORUS ORIENTALIS

Authors:   R.J. Heinrich, F. Klein, A. Hohe
Keywords:   AFLP, Helleborus orientalis, hybrid breeding, inbreeding coefficient
DOI:   10.17660/ActaHortic.2012.961.24
Abstract:
Prerequisite for the development of F1-hybrids in Helleborus orientalis Lam. is the evaluation of potential parentsí inbreeding levels. As no codominant marker system is available, a method to estimate the inbreeding coefficient based on AFLP-markers has been applied and evaluated based on the progeny of a presumable selfing experiment. The effect of marker numbers used in the analysis and the composition of an out-group for this calculation were analysed. Practical problems of the method are discussed.

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