ISHS


Acta
Horticulturae
Home


Login
Logout
Status


Help

ISHS Home

ISHS Contact

Consultation
statistics
index


Search
 
ISHS Acta Horticulturae 961: VII International Symposium on In Vitro Culture and Horticultural Breeding

AN IMPROVED MICRO PROPAGATION SYSTEM FOR SUCCESSFUL TRANSFORMATION OF CAULIFLOWER (BRASSICA OLERACEA VAR. BOTRYTIS)

Authors:   F.G. Al-Swedi, S. Lane, M.P. Fuller
Keywords:   cauliflower, micro propagation, Agrobacterium tumefaciens
DOI:   10.17660/ActaHortic.2012.961.19
Abstract:
Cauliflower is described as a recalcitrant plant to the genetic transformation process from Agrobacterium. Cauliflower curd meristems were used in a fractionation and sieving protocol to produce microshoots in in vitro culture. This technique produced many thousands of small explants suitable for transfection studies. Explants were successfully transformed following standard transfection with A. tumefaciens carrying a construct with KanamycinR, GUS and the Ascorbate Peroxidase (APX) gene. Selection using Kanamycin produced normal plant phenotypic plantlets and these putative transformants were confirmed using GUS staining and PCR of the APX gene sequence. The transfection conditions were optimised during this study and 105 Agrobacterium cells/5 plants/pot successfully produced 1 transformed plant/pot.

Download Adobe Acrobat Reader (free software to read PDF files)

961_18     961     961_20

URL www.actahort.org      Hosted by KU Leuven      © ISHS