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ISHS Acta Horticulturae 96: II International Symposium on Spices and medicinal Plants

APPLICABILITY OF OVERPRESSURED THIN-LAYER CHROMATOGRAPHY IN PHYTOCHEMISTRY

Authors:   E. Tyihák, E. Mincsovics, P. Tétényi, I. Zámbó, H. Kalász
Abstract:
According to some theoretical calculations the quadratic law of thin-layer chromatographic development isn't valid in overpressured thin-layer chromatography. The migration of the solvent front in this new planar liquid chromatographic technique is described by a simple equation. The stable and high speed flow velocity of mobile phase in overpressured thin-layer chromatography gives a good opportunity for the quick and efficient separation of various plant substance groups. These advantages were demonstrated by separation of Digitalis glycosides

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