EVIDENCE OF ANTHELMINTIC ACTION OF AERIAL PARTS FROM INULA VISCOSA AIT. ATTRIBUTION TO A SESQUITERPENIC ACID OF THIS ACTIVITY

Roots and aerial parts from Inula viscosa Ait. are harvested during the blossoming /Montpellier - France/. The tested extracts are separated with several solvents. Sesquiterpenic acid is isolated by column chromatography. Extracts and pure product are tested in vitro on Syphacia obvelata, Hymenolepis nana var. fraterna and Fasciola hepatica. Sesquiterpen acid is tested in vivo.

Data show a real anthelmintic action of aerial parts from Inula viscosa Ait. while roots present no significant activity. In the aerial parts, soluble fractions in water are inactive, the most active is obtained with the means of dichloromethane. A sesquiterpenic acid responsible of the anthelmintic activity has been isolated from this extract. The physical and chemical analysis /UV, IR, NMR, Mass-Spectrometry.…etc./ show an identical structure with a sesquiterpene acid already described, but its activity not seems known. The activity of this acid in vitro is real towards the three genus of worms. Subsequently, a determination in vivo of the anthelmintic activity has been made for sesquiterpenic acid. The obtained results comparatively to products of known reference, reveal that only the aerial parts from Inula viscosa Ait. have an antiparasitic significant activity. Finally, we have verified the absence of alantolactone and isoalantolactone in the roots and aerial parts.

Download Adobe Acrobat Reader (free software to read PDF files)