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ISHS Acta Horticulturae 940: XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on the Challenge for a Sustainable Production, Protection and Consumption of Mediterranean Fruits and Nuts

EX SITU MANAGEMENT OF FIG (FICUS CARICA L.) GENETIC RESOURCES: TOWARDS THE ESTABLISHMENT OF THE MEDITERRANEAN REFERENCE COLLECTION

Author:   B. Khadari
Keywords:   Ficus carica L., ex situ conservation, fig cultivars, genetic diversity, pomological traits, molecular markers
DOI:   10.17660/ActaHortic.2012.940.7
Abstract:
For the management of ex situ plant genetic resources, two main aims have to be reached. First, all accessions should be characterised in order to eliminate cases of mislabelling and redundancies, and to create a complete database to define the reference collection. Second, to assess their genetic diversity regarding in situ genetic resources in order to test whether a germplasm collection is completely or partially representative. The present study aimed to examine these two topics on an ex situ fig collection maintained in the Porquerolles island (Southern France) including 383 accessions from several collections and traditional orchards in different Mediter-ranean countries (807 fig trees). All these 807 fig trees were analysed using 17 SSR loci and 97 alleles were detected. A total of 318 SSR profiles were identified and classified into 269 different genotypes that were distinguished by 4 to 34 alleles and 49 closely related genotypes (distinguished by 1 to 3 SSR alleles). The Bayesian model clustering analysis of Mediterranean cultivated and wild fig populations showed that the Porquerolles collection is largely representative of Mediterranean genetic diversity. Comparing the defined genotypes of this collection to accessions considered as reference of main cultivars based on pomological traits and SSR loci, the complexity of defining reference material for fig cultivars was examined. This study is a frame¬work for the establishment of the Mediterranean reference collection of fig cultivars.

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