ISHS Acta Horticulturae 94: XI International Symposium on Fruit Tree Virus Diseases
STRAWBERRY LATENT RINGSPOT AND CUCUMBER GREEN MOTTLE MOSAIC VIRUSES IN APRICOTS WITH THE BARE TWIG AND UNFRUITFULNESS DISEASE SYNDROME |
| Authors: | M. CECH, M. FILIGAROVÁ, J. POZDENA, H. BRANIŠOVÁ |
| Abstract:
Two morphologically distinct viruses were isolated from apricots (south Moravia) showing “bare twig and unfruitfulness” disease. One of them had isometric particles about 28 nm in diameter. It was freed from the associated rod-like virus by repeated passage through Chenopodium quinoa plants and was identified serologically as strawberry latent ringspot virus (SLRV). The separation of the anisometric virus was achieved by growing inoculated cucumber plants at 28–35°C and fractionating sap extracts by sucrose gradient centrifugation to avoid SLRV. Final elimination of SLRV was accomplished by subsequent thermal inactivation (10 min at 75°C) and dilution near to the end point. One percent of inoculated cucumber plants became infected with the rod-like virus which was found to be closely related to the yellow (aucuba) strain of cucumber green mottle mosaic virus. Both viruses were transmitted back to apricot and peach seedlings. | |
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