ISHS Acta Horticulturae 94: XI International Symposium on Fruit Tree Virus Diseases
SOME PROPERTIES OF A NUCLEIC ACID ASSOCIATED WITH LITTLE CHERRY DISEASE |
| Authors: | R.I. HAMILTON, J.A. DODDS, J. RAINE |
| Abstract:
A nucleic acid was isolated from leaves of sweet cherry (Prunus avium L.) infected with little cherry disease (LCD) by a method which is highly selective for double-stranded RNA (ds-RNA). The nucleic acid was susceptible to RNase in low salt but not in high salt and it was resistant to RNase-free DNase. Its molecular weight, estimated by polyacrylamide gel electrophoresis using the ds-RNA of tissue infected with turnip yellow mosaic virus, as a standard, was slightly greater than 4X106. The ds-RNA was not obtained from sweet cherry known to be free of LCD nor from sweet cherry infected with known viruses. | |
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