ISHS Acta Horticulturae 94: XI International Symposium on Fruit Tree Virus Diseases
SEROLOGICAL DETECTION OF APPLE CHLOROTIC LEAF SPOT VIRUS (CLSV) AND APPLE STEM GROOVING VIRUS (SGV) IN APPLE TREES |
| Author: | E. FUCHS |
| Abstract:
Because of the instability of apple chlorotic leaf spot virus (CLSV) and apple stem grooving virus (SGV) as well as the low concentration of these viruses in apple trees serological detection was only possible with sensitive latex and ELISA methods. The latex test following SCHADE (1971) was used for the routine identification of CLSV in petals in the period from May to June. We succeeded in detecting SGV by means of ELISA in buds of leaves and petals forced in a greenhouse at 18 to 25°C in the period from October to April and in naturally grown petals in May. Fruits proved less suitable as test material. As a result of our experiments we were able the first time to identify SGV during the winter time. | |
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