ISHS Acta Horticulturae 94: XI International Symposium on Fruit Tree Virus Diseases
USE AND VERSATILITY OF THE IMMUNOENZYMATIC ELISA PROCEDURE IN THE DETECTION OF DIFFERENT STRAINS OF AN APPLE CHLOROTIC LEAF SPOT VIRUS |
| Authors: | G. Detienne, R. Delbos, J. Dunez |
| Abstract:
Apple chlorotic leaf spot virus (CLSV) is known to infect most fruit tree species. Frequently more or less symptomless in pome fruits, it appears responsible for serious diseases in stone fruits. Due to the great diversity of strains, biological detection of the virus is sometimes difficult. Application of the ELISA test was recently described by FLEGG and CLARK. According to these authors the double antibody sandwich procedure failed to detect CLSV and they presented a modification of the established procedure suitable for the detection of this virus. In the course of our work we observed that the established double sandwich method can be used if special extraction buffers are employed. Our purpose was to compare the advantages of the biological indexing and the ELISA tests and to check their respective specificities for detecting various of CLSV strains. The ELISA test allowed the detection of 9 different strains, including pear ring mosaic, isolated from 13 fruit tree species. As a result of our investigations we found that serological detection with the ELISA procedure is less discriminating than biological indexing. | |
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