ISHS Acta Horticulturae 94: XI International Symposium on Fruit Tree Virus Diseases
SOME PROPERTIES OF TOBACCO NECROSIS VIRUS ISOLATED FROM PLUMS |
| Authors: | K. PAULECHOVÁ, H. BAUMGARTNEROVÁ |
| Abstract:
This paper describes properties of an isolate of tobacco necrosis virus (TNV-B2) from plum (Prunus domestica cv. Bystrická). The virus in sap of Chenopodium quinoa had a dilution end point of 10-8, a thermal inactivation point of 85°C, and longevity in vitro at room temperature of 8 weeks. Virus purified by differential centrifugation contained 19.5–20.0% RNA and had an absorption spectrum with a maximum at 260 nm and minimum at 240 nm. The ratio of virus absorption at 260/280 nm was 1.5. A specific antiserum was prepared which reached a titer of 1 : 256 in double agar gel diffusion tests and 1 : 512 in drop precipitation tests. Purified preparations contained isometric particles of 28–30 nm diameter. The purified preparations reacted similarly with homologous antiserum and with antiserum to TNV-RV. | |
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