ISHS Acta Horticulturae 91: Symposium on Growth Regulators in Floriculture
BIOLOGICAL ACTIVITY OF ENDOGENOUS AND INFLUENCE OF EXOGENOUS GROWTH REGULATORS ON CANNA INDICA REGENERATION IN VITRO |
| Author: | K. Kromer |
| Abstract:
The influence of growth regulators on formation of callus, root and buds in tissue explants was investigated in vitro culture. Last year growth of Canna indica rhizomes was taken as material. The medium MS was used with addition of various auxins /NAA, IAA and 2,4-D/ and kinetin. At the same time the biological activity of endogenous growth regulators in examined tissues was investigated. The low activity of auxin-like and gibberellin-like substances was observed. The growth inhibitors of ABA type showed average activity while cytokinin-like substances showed high activity. Formation of organs did not take place on the basic medium MS in vitro cultures. Addition of auxin only gave but a poor formation of shoot buds. Kinetin /1 ppm/, added to MS medium containing the auxin stimulated the formation of shoot buds and plants. The best effects were achieved on MS medium with 2 ppm IAA and 1 ppm kinetin. | |
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