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ISHS Acta Horticulturae 901: XII International Symposium on Virus Diseases of Ornamental Plants

VALIDATION OF PLANT VIRUS DETECTION

Authors:   A.R. van Schadewijk, E.T.M. Meekes, M. Verbeek, J.T.J. Verhoeven
Keywords:   accreditation, performance characteristics, ELISA, PCR
Abstract:
Validation of test methods is required for laboratories seeking ISO 17025 accreditation. Recently developed manuals help choosing relevant performance characteristics to be studied for qualitative tests common in plant virus detection. For routine testing in certification schemes additional information on the plant material is important to determine the scope of the accredited application. For application of virus tests for diagnostic purposes the determination of certain performance charac-teristics is highly recommended. Furthermore, in case of detection of quarantine organisms a confirmation by an alternative test is indispensable.
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