|Authors: ||M.W. Dees, C. Spetz, D.-R. Blystad|
|Keywords: ||Potexvirus, soil sample, Tulip virus X|
A virus (isolate 10306) was isolated from bait plants (Nicotiana sp.) grown in a soil sample collected from an old orchard in the eastern part of Norway.
Preliminary results from electron microscopy identified isolate 10306 as a putative member of the genus Potexvirus based on particle morphology.
Molecular and biological methods were further used to determine the coat protein (CP) sequence and the host range of isolate 10306, respectively. Potexvirus-specific degenerate primers were designed and used to amplify a ~300 nucleotide fragment belonging to the RNA-dependent RNA-polymerase region of the viral genome by reverse-transcription PCR (RT-PCR). Based on the results from the sequence analysis, new specific primers were designed to amplify a fragment encompassing the CP gene.
Furthermore, isolate 10306 was mechanically inoculated to different test plants and found to systemically infect eight plant species belonging to five families.
CP sequence comparisons, at amino acid and nucleotide level, as well as phylogenetic analysis identified isolate 10306 as Tulip virus X (TVX). However, results from the host range studies differed from the ones previously published for this virus, suggesting that isolate 10306 could be considered a different strain of TVX. Moreover this is the first report of TVX in Norway.
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