ISHS Acta Horticulturae 901: XII International Symposium on Virus Diseases of Ornamental Plants
CHARACTERIZATION OF A SEVERE STRAIN OF CHRYSANTHEMUM VIRUS B (CVB) ISOLATED FROM CHRYSANTHEMUM IN INDONESIA |
| Authors: | I.G.R.M. Temaja, G. Suastika, S.H. Hidayat, U. Kartosuwondo |
| Keywords: | Chrysanthemum virus B (CVB), chrysanthemum (Chrysanthemum morifolium), Macrosiphoniella sanborni (chrysanthemum aphid) |
| Abstract:
Infection of Chrysanthemum virus B (CVB) has been reported from many countries where chrysanthemum plants are cultivated. An isolate of CVB obtained from a chrysanthemum (Chrysanthemum morifolium) plantation in Indonesia was characterized and found to be unique. The virus isolate, designated as CVB-Ina, shared similar characters with other CVB isolates based on electron microscopy analysis and serological reactions. However, the results of bioassay showed that CVB-Ina was very distinct from most CVB isolates reported earlier. CVB-Ina induced vein banding and mottling on the leaves of chrysanthemum plants, could infect Nicotiana benthamiana, and was transmitted by the chrysanthemum aphid, Macrosiphoniella sanborni. In contrast, most CVB isolates were not able to induce any symptoms on chrysanthemum leaves, were not able to infect N. benthamiana, and were transmitted at low efficiency by the aphids. Furthermore, molecular characterization of the coat protein gene exhibited some differences in amino acid sequences. This might explain the distinct biological characteristics of CVB-Ina. | |
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