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ISHS Acta Horticulturae 901: XII International Symposium on Virus Diseases of Ornamental Plants

EXPRESSION OF THE CHRYSANTHEMUM VIRUS B COAT PROTEIN GENE IN TRANSGENIC CHRYSANTHEMUM

Authors:   T. Mitiouchkina, T. Skachkova, O. Shulga, S. Dolgov
Keywords:   Chrysanthemum morifolium Ramat., virus resistance, coat protein, genetic transformation, Chrysanthemum virus B, antisense RNA
Abstract:
The most promising strategy to create virus resistant plants today is gene silencing technology called RNA interference. The essence of RNA-induced gene silencing is the delivery of double-stranded RNA into an organism or cell, inducing a sequence-specific RNA degradation mechanism that effectively silences a targeted gene. For this purpose we transferred the Chrysanthemum virus B coat protein gene sequences into specific vector pKannibal in order to generate small double stranded RNA complementary to this sequence and obtained four transgenic lines of chrysanthemum ‘White Snowdon’.
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