ISHS Acta Horticulturae 897: V International Symposium on Banana: ISHS-ProMusa Symposium on Global Perspectives on Asian Challenges
EARLY EVENTS IN THE FUSARIUM OXYSPORUM F. SP. CUBENSE ¿ MUSA INTERACTION USING GREEN FLUORESCENT PROTEIN-TAGGED RACE 4 |
| Authors: | C.Y. Li, G.J. Yi, B.Z. Huang, Y.R. Wei |
| Keywords: | banana, Fusarium wilt, green fluorescent protein |
| DOI: | 10.17660/ActaHortic.2011.897.42 |
| Abstract:
Fusarium oxysporum f. sp. cubense (Foc) is the causal pathogen of Fusarium wilt of banana. To understand some of the early events in the Foc-Musa interaction, we created a green fluorescent protein (GFP)-tagged transformant and studied its pathogenesis using fluorescence microscopy and confocal laser scanning microscopy. The study showed that: (i) Foc race 4 was capable of invading the epidermal cells of banana roots directly; (ii) Invasion sites included epidermal cells of root caps and in the elongation zone. Our results showed that roots of Foc race 4-susceptible banana plants were well colonised with the pathogen and demonstrated that GFP-tagged Foc race 4 isolates present an effective tool to study banana root-fungus interactions. | |
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