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ISHS Acta Horticulturae 896: XII International Workshop on Fire Blight

DETECTION OF ERWINIA AMYLOVORA BY PCR WITH PRIMERS TO THE HRPN GENE

Authors:   A.L. Lagonenko, V.Y. Lagonenko, Y.A. Nikolaichik, A.N. Evtushenkov
Keywords:   Erwinia amylovora, fire blight, PCR, hrpN, diagnostics
DOI:   10.17660/ActaHortic.2011.896.9
Abstract:
A molecular diagnostic method for detection and identification of the fire blight pathogen Erwinia amylovora, based on polymerase chain reaction (PCR) amplification of a 1200-bp fragment of the chromosomal gene hrpN was developed. The specific primers to the harpin gene amplified DNA from 17 strains of E. amylovora isolated in four countries from different plant hosts, but not from 25 strains of other species of plant-pathogenic and saprophytic bacteria. The observed sensitivity of the diagnostic procedure was 10 E. amylovora cells per PCR reaction.

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