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ISHS Acta Horticulturae 896: XII International Workshop on Fire Blight

GENETIC CHARACTERIZATION OF BELARUSIAN ERWINIA AMYLOVORA STRAINS

Authors:   A.L. Lagonenko, I.V. Kudzina, A.N. Evtushenkov
Keywords:   Erwinia amylovora, molecular differentiation, pEA29 RFLP, rep-PCR, RAPD PCR, PCR-ribotyping
DOI:   10.17660/ActaHortic.2011.896.18
Abstract:
In this work we report the preliminary genetic characterization of 11 Erwinia amylovora strains, isolated from apple and pear trees in different regions of Belarus in comparison with several authentic strains from Poland, Germany and Great Britain. Two strains isolated in Poland showed the lowest virulence on immature pear fruits. Strains were analyzed by RFLP of the pEA29 PstI amplified fragment, rep-PCR, RAPD and PCR-ribotyping techniques. All tested E. amylovora strains except 1/79 had 5 repeats in the SSR array on pEA29 plasmid. REP, ERIC and PCR-ribotype DNA fingerprints were identical in all cases. PCR-ribotype 3 was detected among all E. amylovora strains. Using RAPD PCR, DNA polymorphism was found within the strains, but Belarusian isolates were undistinguishable from E. amylovora isolates from other countries.

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