ISHS Acta Horticulturae 894: I International Symposium on Tropical Horticulture
STUDY ON THE GENETIC RELATIONSHIP OF 35 CAVENDISH BANANA (MUSA AAA) VARIETIES BY RAPD MOLECULAR MARKER |
| Authors: | H.B. Chen, Q.J. Wang, Y.H. Qin, G.A. Zhang, G.B. Hu |
| Keywords: | Cavendish banana (Musa AAA), RAPD, identification, genetic relationship |
| DOI: | 10.17660/ActaHortic.2011.894.9 |
| Abstract:
In this study, the RAPD (random amplified polymorphic DNA) technology was used to identify the genetic relationship of 35 Cavendish banana (Musa AAA) varieties (26 from China, 3 from ITC, 2 from Philippines, 1 from Thailand, 1 from Australia, 1 from Mexico and 1 from Somalia). A suitable reaction system of RAPD for bananas was established, which included 1.2-fold 10×buffer, 0.2 mM dNTP, 0.25 μM primer, 1.25 U Taq DNA polymerase, and 0.8 ng/μl DNA templates in 25 μl reaction volume. Using above optimal conditions, genetic relationship of 35 Cavendish banana varieties was further screened out. 890 fragments were produced by using 107 primers selected from 1,200 random RAPD primers. NTsys software was used for data analysis and respective dendrogram depicting Jaccard’s similarity coefficients by using UPGMA method. The 35 Cavendish banana varieties had very close genetic relationship. The highest similarity coefficient was 0.922 while the lowest was 0.850. The results indicated that RAPD technology is applicable to identifying different banana varieties and can serve as an effective tool for exploring the banana genetic relationship. | |
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